To investigate the changes in oxygen consumption rate and suffocation point of Eleutheronema tetradactylum under different water temperature conditions, the experiment compared the effects of different temperatures (22,27,32 ℃) on the oxygen consumption of E.etradactylum with an average length of (4.5 ± 0.5) cm and an average body mass of (1.5 ± 0.5) g.The experimental results show that: 1) Under the conditions of this experiment, the higher the water temperature, the shorter the time for all juvenile fish to die, and the higher the mass concentration of dissolved oxygen (DO) in the water. The suffocation point at 22 ℃ is 1.39 mg/L, the suffocation point at 27 ℃ is 1.54 mg/L, and the suffocation point at 32 ℃ is 1.76 mg/L; 2) The oxygen consumption rate of juvenile fish in each temperature group showed a decreasing trend with the extension of experimental time, and the higher the temperature, the faster the decrease rate of oxygen consumption rate. When the oxygen consumption rate decreased from 1.418, 2.346, and 4.243 mg/(g·h) at the beginning of the experiment to 0.4-0.5 mg/(g·h), all juvenile fish died, and the changes in different temperature groups slowed down. The concentration difference between adjacent observation points was not significant (P>0.05), while the survival rate of juvenile fish in the control group was 100%; 3) During the experiment, when the DO content of each temperature group was 2.53, 2.90, and 3.10 mg/L, the juvenile fish began to exhibit disorderly movement, restlessness, occasional jumping out of the water, and gill opening. As the experiment continued, the frequency of the juvenile fish jumping out of the water increased, and the amplitude of gill cover vibration increased accordingly. After intense stress, they lay still at the bottom of the water, twitching and suffocating to death.